Taken with a Canon T2i/550D and Canon 100mm f/2.8 Macro lens at f/5.6. 62 1/30-second exposures at ISO100 in a focus stack (DoF stack) were combined with Zerene Stacker using DMap mode. Shooting was done tethered to a PC using the Canon EOS utility. Focus stepping was done by pressing the live view ">" focus button once between each exposure in the stack.